Molecular Microbiology Diagnostic Principles And Practice Pdf


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Capturing rapidly evolving molecular medical microbiology

In , the American Society for Microbiology Press decided it was time to publish an update on molecular microbiology. The third edition of Molecular Microbiology: Diagnostic Principles and Practice reflects the changes that have been made in the field since the last edition was published in In 5 years, molecular techniques have evolved rapidly and their use in diagnosing infectious disease has become widespread.

For example, point-of-care tests have increasingly been used to detect resistance genes in routine laboratory tests, and the first next-generation sequencer has been approved by the US Food and Drug Administration FDA. Describing the principles and illustrating the practical use of molecular microbiology in infectious diseases in a single volume is a monumental undertaking. The editors invited more than authors to achieve this feat in 12 sections.

The book begins by looking at the use of molecular microbiology in health-care-associated infections and in public health, with more technical topics, such as information technology, quality assurance, and the business of diagnostics, discussed later.

Information Technology and The Business of Diagnostics are new sections in this edition. In Information Technology, authors discuss point-of-care device connectivity and surveillance software, broadening readers' undestanding about molecular testing in medical microbiology and infection control fields. Somewhat confusingly, research-oriented sections, such as metagenomics ie, studies of the microbiome , and the host and host response are separated. Perhaps, the editors wanted to emphasise that the microbiome is a hot topic by placing this section towards the front.

The chapters of the book are generally concise, while providing enough in-depth discussion for readers to understand the topic. Commendably, despite a large number of authors from Europe, the USA, and China, the style of the chapters is homogenous.

We especially like that many of the chapters conclude by giving future perspectives, given that at this moment, it is difficult to predict whether molecular diagnostics will overtake current microbiology in the laboratory, or rather have a supportive role.

Furthermore, it is interesting to read the authors' predictions on which particular platform or methods will have a dominant role in next-generation sequencing. A nice surprise in the book is a chapter on the effect of molecular diagnostics on public health in China. The book is likely to be of interest to all people involved in the diagnosis and research of infectious diseases. For clinical microbiologists and infectious disease specialists, it provides an overview of the molecular techniques used to diagnose infectious disease, from PCR to mass spectrometry.

For readers who are new to the field, clear explanations are provided for methods such as 16S ribosomal rRNA sequencing ie, why and how this method is used in bacteria identification.

For national reference centers, up-to-date information about specific pathogens is provided, such as for methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. The book also offers laboratory managers advice on how to design a clinical microbiology laboratory taking into consideration recent advances eg, point-of-care tests. Especially interesting for researchers are the chapters discussing the the pros and cons of different next-generation sequencing platforms.

Although the editors-in-chief David Persing and Fred Tenover work at a molecular diagnostics company, the book provides balanced reviews of commercial assays from various manufacturers. These comparisons are presented in table format, offering readers an accessible overview of which assay is most suitable to use. However, this third edition could have been improved in a few ways. For readers not familiar with molecular technique terms, a glossary of terms would have been beneficial. Furthermore, information could have been provided more succinctly, with less overlap between chapters on the principles of next-generation sequencing platforms.

We also believe that a separate chapter on carbapenemase-producing microorganisms, which are a major public health problem worldwide, should have been included. Finally, it should be noted that although techniques and microorganisms are discussed comprehensively, the book is not intended to be exhaustive; for more in-depth information, specific books should be consulted. Despite these limitations, this book is likely to be a valuable reference for people involved in the diagnosis, treatment, and surveillance of infectious diseases, and who are interested in molecular techniques.

National Center for Biotechnology Information , U. Lancet Infect Dis. Published online Mar Reviewed by Erlangga Yusuf and Herman Goossens. Copyright and License information Disclaimer. All rights reserved. Elsevier hereby grants permission to make all its COVIDrelated research that is available on the COVID resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source.

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Molecular Microbiology: Diagnostic Principles and Practice

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Persing, Fred C. Nolte, Randall T. Hayden, and Alex Van Belkum pages. I found Molecular A considerable number of chapters are devoted to teaching Microbiology: Diagnostic the reader how to analyze the data generated by the Principles and Practice, 2nd various molecular platforms. Illustrations are plentiful and Edition to be an excellent well-chosen; they provide clarity to the written text. Another resource for laboratory important detail included by the authors is information professionals and the pertaining to functional design of a molecular laboratory clinical care team. This book space.

In , the American Society for Microbiology Press decided it was time to publish an update on molecular microbiology. The third edition of Molecular Microbiology: Diagnostic Principles and Practice reflects the changes that have been made in the field since the last edition was published in In 5 years, molecular techniques have evolved rapidly and their use in diagnosing infectious disease has become widespread. For example, point-of-care tests have increasingly been used to detect resistance genes in routine laboratory tests, and the first next-generation sequencer has been approved by the US Food and Drug Administration FDA. Describing the principles and illustrating the practical use of molecular microbiology in infectious diseases in a single volume is a monumental undertaking. The editors invited more than authors to achieve this feat in 12 sections. The book begins by looking at the use of molecular microbiology in health-care-associated infections and in public health, with more technical topics, such as information technology, quality assurance, and the business of diagnostics, discussed later.

Molecular microbiology: diagnostic principles and practice.

Par carruth claudette le lundi, avril 3 , - Lien permanent. Difficile, the Xpert for the diagnosis of C. Developing and Validating Diagnostic Assays for Biodefense. Difficile, and its use is supported by the most recent practice guidelines nm on the Biowhittaker microplate reader Biowhittaker Molecular Applications, Diagnostic principles and practice, 2nd ed. Persing, Ed.

The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.

The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in

Molecular Microbiology: Diagnostic Principles and Practice, 3rd Edition

American Society for Microbiology, Used Good. Buy, rent or sell. Principles of Molecular Diagnostics and Personalized Cancer Medicine The role of molecular genetics in the treatment of malignancy continues to grow at an astonishing rate. Todayus subspecialized multidisciplinary approach to oncology has incorporated advances in targeted molecular therapy, prognosis, risk assessment, and preventionall based at least in part on molecular An introduction to microbiology, aseptic technique and safety Preparation Safety guidelines 1 Risk assessment 2 Good microbiological laboratory practice GMLP 3 Spillage management 3 Aerosols 3 Resources Equipment 4 Apparatus 5 Materials 5 Media, sterilisation and disinfection Preparation of culture media 6 Pouring a plate 6 Storage of media 6. Each of the following statements concerning the Gram stain is correct except a.

I found Molecular Microbiology: Diagnostic Principles and Practice, 2 nd Edition to be an excellent resource for laboratory professionals and the clinical care team. This book provides chapter-by-chapter detail regarding the various molecular techniques that have been incorporated into use in microbiology laboratories during the past 30 years. The authors focus on molecular techniques for routine use in clinical and research laboratories. The work includes comprehensive functional and economic comparison of currently available commercial reagents, as well as discussion of specialized laboratory developed methods. A considerable number of chapters are devoted to teaching the reader how to analyze the data generated by the various molecular platforms. Illustrations are plentiful and well-chosen; they provide clarity to the written text. Another important detail included by the authors is information pertaining to functional design of a molecular laboratory space.

Bailey & Scott's Diagnostic Microbiology, 12th Edition (Diagnostic Microbiology (Bailey & Scott's))

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